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184B5
  • 平台编号:bio-68279
  • 规格:0.5mg
  • 拉丁属名:
  • 购买数量:
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ATCC Number:CRL-8799?
相关疾病:正常
生长状态:贴壁生长
器官来源:乳房
组织来源:epithelium
数量:大量
细胞类型:其他细胞类型
是否是肿瘤细胞:0
物种来源:人
运输方式:冻存运输
细胞形态:上皮样
年限:21 years
规格:0.5mg Designations: 184B5
Depositors: ?The United States of America
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial


Source: Organ: mammary gland; breast
Tissue: epithelium
Disease: normal
Cell Type: chemically transformed
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:No
Cytogenetic Analysis:47, XX
Age: 21 years
Gender: female
Comments:The 184B5 cell line was established from normal mammary tissue obtained from a normal reduction mammoplasty.
Cells derived from the tissue were exposed to benzo(a)pyrene, and a transformed line was established.
The line appears to be immortal, but is not malignant.
When seeded at low density, the cells grow in tightly packed colonies.
Propagation: ATCC complete growth medium: The base medium for this cell line (MEBM) along with the additives can be obtained from Lonza/Clonetics Corporation as a kit: MEGM, Kit Catalog No. CC-3150. ATCC does not use the GA-1000 (gentamycin-amphotericin B mix) provided with kit. To make the complete growth medium, you will need to add the following components to the kit (sold separately):
  • 1 ng/ml cholera toxin
  • Note: Do not filter complete medium
    Temperature: 37.0°C
    Subculturing: Protocol:
    • Remove and discard culture medium.
    • Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution.
    • Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
      Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
    • Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
    • To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 xg for 5 to10 minutes.Discard supernatant and resuspend cells in fresh serum-free growth medium. Add appropriate aliquots of cell suspension to new culture vessels.
    • Place culture vessels in incubators at 37?C.

    Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
    Medium Renewal: Every 2 to 3 days
    Preservation: Freeze medium: Complete growth medium suplemented with 10% (v/v) fetal bovine serum and 5% (v/v) DMSO
    Storage temperature: liquid nitrogen vapor phase
    References: 21894: . Transformation of human epithelial cells. Boca Raton, FL: CRC Press; 1992.
    21926: Stampfer MR. Continuous human cell lines and method of making same. US Patent 4,808,532 dated Feb 28 1989
    22413: Walen KH, Stampfer MR. Chromosome analyses of human mammary epithelial cells at stages of chemical-induced transformation progression to immortality. Cancer Genet. Cytogenet. 37: 249-261, 1989. PubMed: 2702624
    23289: Stampfer MR, Bartley JC. Induction of transformation and continuous cell lines from normal human mammary epithelial cells after exposure to benzo[a]pyrene. Proc. Natl. Acad. Sci. USA 82: 2394-2398, 1985. PubMed: 3857588
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